Cell culture

Sterile Techniques, Tissue Culture and Cell Counting
Version 8-18-12 • Tissue culture is a technique used by scientists to grow cells from various tissues in a. laboratory setting. • In this experiment you will propagate your own cell line for a number of weeks, conducting weekly propagation as well as quantifying and characterizing your cell culture. • ... In future labs you will determine the average protein concentration of the cells in your culture (using previously prepared protein standard curves), as well as assess the success of your cryopreservation technique. •
Cell suspensions at a given concentration
LABORATORY ONE: Cell Culture Techniques. The development of tissue or cell culture has had a profound effect on the study of animal virology. Cell cultures are relatively easy to maintain and allow the virologist to study a virus of interest in an environment which can be controlled and manipulated fairly easily. Many types of cell lines have been developed which make it possible for most animal viruses to be studied in cell culture. In the introductory lecture, the lab instructor will describe the defined cell culture medium that makes cell culture possible.
12 October
LAB 5 Mammalian Cell Culture
1. Demonstrate excellent aseptic technique 2. Set up and use a Biological Safety Cabinet (BSC) for performing cell culture. techniques 3. Operate an inverted light microscope 4. Estimate percent confluence of a cell culture 5. Determine the density of a cell culture using a hemocytometer 6. Dilute a cell culture for seeding 7. Maintain a cell culture for several weeks by passing the cells regularly. TIMELINE: This lab will be continued through the first half of the semester.
Lab Exercise 5: Pure culture techniques
Bacillus anthracis. contributed to many of the laboratory techniques we still use today, including the method for isolating pure c ul tures of bacteria. The most commonly used method in the laboratory for isolating microbes is the s trea k pla te, and to a lesser extent, the po ur pla te. ... The pure colony is essentially a clone of cells that all are identical copies of the original cell and can be used for further study. Lab Exercises. I. Streak plate method. Table supplies 1 mixed culture of. Serratia marcescens, Escherichia coli
Workshop on Eukaryotic Cell Culture Techniques
Course Outline The workshop includes informal lectures and hands-on experience on basic eukaryotic cell culture techniques. Lecture’s Topics §Introduction to primary cell culture. § ... Biosafety measures for primary cell culture. Lab Exercise §Isolation of peripheral blood mononuclear cell s (PBMCs) from whole blood. § Culturing PBMCSs . § Thawing and cryopreservation of PBMCs §Cell counting and viability determination.
Cell culture and stem cell techniques | Doug Bruce
In early July, in the midst of teaching my summer biomanufacturing course, the North Valley Biotechnology Center (NVBC) announced a two-week intensive cell culture and stem cell techniques course with Dr. Jason Bush at Fresno State University. I leapt at the opportunity and was selected to join five other students and faculty from around California to participate ... My cell culture skills needed some brushing up, and I have never had the opportunity to work with stem cells. I previously worked in a neuro-oncology research lab doing cell culture and fluorescent in-situ hybridization (FISH) of cancer cell chromosomes.
19 October
lab4pureculture
In this laboratory exercise, you will be learning the pour plate and streak plate techniques. Both of these techniques depend upon the physical/spatial isolation of a single bacterial cell on/in a solid medium. ... Aseptic techniques not only protect a laboratory culture from becoming contaminated, but also protect the worker and the environment from becoming contaminated by the microorganisms. You should be able to perform standard aseptic techniques with ease and precision so that isolation and cultivation of bacteria in pure culture may be accomplished.
21 June
Basic Techniques for Mammalian Cell | Cell Culture
Cell cultures are utilized in cytogenetic, biochemical, and molecular laboratories for diagnos-tic as well as research studies. In most cases, cells or tissues must be grown in culture for days or weeks to obtain sufficient numbers of cells for analysis. Maintenance of cells in long-term culture requires strict adherence to aseptic technique to avoid ... Some labs prefer to split the cells 1:3 or 1:4, although increasing the split ratio will result in a longer interval before subcultures reach confluency. Freezing human cells grown in monolayer cultures. It is sometimes desirable to store cell lines for future study.
Cell culture basics | Cell Culture Laboratory 4
The handbook and videos are intended as an introduction to cell culture basics, covering topics such as getting familiar with the requirements of a laboratory dedicated to cell culture experiments, laboratory safety, aseptic technique, and microbial contamination of cell cultures, as well as providing basic methods for passaging, freezing, and thawing cultured cells. ... Biosafety Level 2 (BSL-2) BSL-2 is appropriate for moderate-risk agents known to cause human disease of varying severity by ingestion or through percutaneous or mucous membrane exposure. Most cell culture labs should be at least BSL-2, but the...
Laboratory Techniques
Department of Biological Sciences
This requires learning and developing techniques for cell isolation and in vitro cell culture; aseptically and in disease free conditions. In order to remain competitive we must learn these techniques as soon as possible so that we can join the workforce as producers, researchers and educators. ... Lecture Topic. 1. Introduction: Background and advantages of cell and tissue culture, and biology of cultured cells. 2 Design and layout for a dedicated cell culture lab.
Protocol for Disinfection of Cell Culture and
Decontamination of cell culture waste. Cell culture has become a common laboratory technique indispensible to current research. ... Place disinfected tips in Biological Waste Sharps Container. Disinfection of contaminated glassware. 1. Wear the appropriate gloves, lab coat and eye protection whenever using chemical disinfectants. 2. When needed for glassware decontamination, prepare the appropriate microbicidal solution as in bleach, prepare a 10% v/v solution.
06-156 (3-D Cell Culture)
The research, spearheaded by bioengineer Diane Hoffman-Kim, adds to a growing body of evidence that lab culture techniques dramatically affect the way these cells behave. ... “More and more, we’re seeing evidence that cells cultured in three dimensions look and behave more like cells in your body,” said Diane Hoffman-Kim, the Brown bioengineer who spearheaded the new study, “so culture method is critical. If you want to better understand how the human body behaves or how new drugs might fight disease, 3-D may be a better bet.”
12 November
Biotechnology Cell and | BIOT205 – Cell and Tissue Culture
Cell Culture Technicians • work in research and development companies and laboratories to grow bacteria, plant and animal cells in culture flasks and plates using aseptic techniques. • operate and maintain basic laboratory equipment such as centrifuges, pH meters, analytical balances, laminar flow hoods, spectrophometers, light microscopes, and C02 incubators. • prepare cell growth media, reagents, buffers, and stains following standard operating procedures (SOPs).
Laboratory techniques and molecular biology
: Registration : Introductory Lecture : Stem Cell Biology and Mesenchymal. Stem Cells (MSCs) : Technical Properties of the Stem Cell Culture. Lab.and Aseptic Conditions and Basic Stem Cell Laboratory Techniques: Primary, suspension, monolayer and 3D cultures : Isolation of stem cells from bone marrow and adipose tissue for clinical applications.
Course Title: Intro to Tissue Culture Lab
semester to maintain cell cultures. Course Description: Introduction to Tissue Culture is a course designed to provide students with hands-on experience. in the proper laboratory methodology and techniques associated with various cell and tissue cultures. The purpose of. ... There is a subfolder called "Class Protocols on Working with Cells" that contains each protocol. FYI: Math Reference: Basic Lab Calculations for Biotech, Chapter 21 “Common Calculations Relating to Animal Cell Culture Techniques”: pgs 351-365.
Lab 3
STERILE TECHNIQUES. Lab 3. Introduction. ... Diluted disinfectants can be used in the cell culture laboratory for routine hygiene and disinfection of used items of equipment and surfaces in rooms and cabinets. i.Aldehydes: Formaldehyde and glutaraldehyde. Formaldehyde is used at about 4%, and is conveniently prepared by diluting 40% formaldehyde solution (formalin).
Chapter 4 | Sterile technique
This lab will introduce you to standard techniques used in microbiology. Very similar techniques are used to culture yeast and bacteria, although the culture conditions are optimized for each organism. ... In this lab, you will prepare stock cultures of S. cerevisiae met mutant strains on streak plates, quantify the number of viable cells in cultures of S. cerevisiae and S. pombe using spot plates, and use light scattering to quickly estimate cell densities of these same yeast cultures.
Understanding and Managing
Cell culture media, especially unopened bottles of media that are outdated or no longer used in the lab (as long as they do not contain any antibiotics) can provide a very rich, readily available and useful substitute for standard microbiological media. A small amount of serum (3 to 5 ... 17. Gabridge, M. G., and Lundin, D. J. Cell Culture User’s Guide to Mycoplasma Detection and Control. Bionique Testing Laboratories, Saranac Lake, NY (1989). 18. McGarrity, G. J., Sarama, J. and Vanaman, V. Cell Culture Techniques. ASM News 51:170-183 (1985). 19. Gartler, S. M. Genetic Markers as Tracers in Cell Culture.
2009 Lab 8 - CellBiology
A general tutorial introduction to cell culture techniques in Cell Biology. Before beginning any research: You should be aware and understand the legal and ethical issues related to research practice.
5 August
course notebook chapter eight
FGRS: course notebook - chapter eight. Aseptic Technique & Cell Culture. ... Adapted notes from course textbook on starting an overnight culture (small scale): 1.Prepare 10 mL of YPD (from lab stock) in a sterile test tube. 2.Follow the instructions for proper sterilization of the inoculating loop. 3.With a cooled loop or sterile toothpick, scoop a small portion of a single isolated colony (solid stock).
7 March
Tissue Culture Techniques | Disposal of infected media/cells
Plating. Growth Techniques. Maintenance / back up culture. Expansion. Antibiotics. Preface. All human tissue culture work should be carried out wearing gloves and long-sleeved gown in a biosafety cabinet contained in an appropriate area. ... Most major suppliers of culture reagents and media also stock RPMI. Other media types are sometimes used for specific applications for example SFM (Life Tech) for growing hybridomas for antibody purification; DMEM for some mouse lines; Iscoves for chicken cell culture.
1 March
Laboratory Techniques I
• 1952 – development of continuous human tumor cell line. • HeLa. • Uses of cultured cells: • Development of antiviral vaccines • Production of monoclonal antibodies • Production of cell products. • Insulin, HGH, interferon. • Understanding of neoplasia. ... • Aseptic technique – designed to create a barrier between the sterile cell culture & microorganisms in the environment. • Sterile work area • Good personal hygiene • Sterile reagents & media • Sterile handling. Summary -­? I. • Cell & molecular techniques allow scientists to examine subcellular components.
Tissue Culture Detailed Protocol
Proper aseptic technique seeks to eliminate these contaminants. It is important to establish and maintain aseptic techniques particularly given the importance of cell culture to everyone’s experiments in the lab. Contamination can be minor and confined to one or two cultures, can spread among several cultures and infect a whole experiment, or can be widespread and wipe out your entire stock.
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CU Information Package/Course Catalog
Viral isolation techniques, cell culture, embryonated chicken eggs. Reading resources and lecture notes about the subject. Verbal lecture, presentation, discussion. 5. Preparation of cell culture, the tubes, plates and bottles used in the cell production, media used in the cell culture production. ... Cytopathic effects of viruses in cell culture (pyknosis, syncytia etc.) Interferens, haemadsorption, hemagglutination. Laboratory Preparation.
20 September
Lab 7. Media preparation and sterilization, Aseptic technique & pure culture concept. Introduction. In natural environments, microorganisms usually exist as mixed populations. However, if we are to study, characterize, and identify microorganisms, we must have the organisms in the form of a pure culture. ... This method of preventing unwanted microorganisms from gaining access is termed aseptic technique. For the most part, bacterial physiology only can be studied in pure cultures. The best way to obtain a pure culture is to start with a single bacterial cell. This cell then divides quickly, and may...
PPTX
M.sc. biotechnology | BT 1.1: cell biology
05. Animal cell culture techniques, Ed. Martin Clynes, Springer. 06. Plant Cell, Tissue and Organ Culture, By Reinert, J. and YPS Bajaj (Springer – Verlag). ... Biotechnology: A laboratory course by Becker, J.M. BT 3.6: lab-vi: animal cell culture and environmental biotechnology. 01. Preparation of animal cell culture media and membrane filtration.
University of Pittsburgh: Pitt Magazine
Using a homemade cell-culture medium fashioned from the blood of chickens, special salts, and placentas, the Geys tried to deceive countless human cells into believing they were in a live body instead of a lab. Though some cells showed promise, all eventually died. ... Today, cell- and tissue-culture techniques dominate fields ranging from virology to oncology to endocrinology, a breadth that grew largely from the teachings of early scientists like Gey.
14 December
Major Topics to be Included
Provides students with advanced laboratory skills needed for employment in the biotechnology industry. Focuses on use of basic and specialized lab equipment and techniques such as solution chemistry, cell culture, DNA extraction and analysis, and protein extraction and analysis. Emphasis is on lab safety, documentation, quality control, and use of standard operating procedures.
Brain Cell Culture and Imaging: A Laboratory Course
Providing students an opportunity to experience, both conceptually and practically, the power of modern cellular imaging techniques is fundamental to the education of students in the biological sciences. ... - Overview of Xenopus development/staging - type of nerve cell cultures - general principles of cell cultures - basic skills to characterize cell cultures (cell morphology, immunohistochemical staining, immunoblotting) - Specifics on Xenopus culture. - Lab – 354 Bardeen · Lab tour · Watch Xenopus dissection video (?)
Basic bacteriological techniques
Aseptic technique reduced the potential spread of bacteria used in laboratory to you or other people. This could happen if a pathogen were allowed to escape the lab on contaminated clothing, books, or other materials. ... Remarkably, when bacteria are collected from a site (such as by swabbing a surface) and transferred to standard bacterial culture media, only a small fraction of the cells present will actually grow. Those that do grow are abundant enough, but most species have specialized growth requirements that are not met by standard culture media.
Advanced Molecular and Cell Culture Techniques. Study level. Second degree. ... Knowledge. Realized aim of course is to acquaint students with basic information about methodology of molecular and cellular research, design of experiments with animal cell cultures, designing laboratory analyses. Skills. On successful completion of the course student will be able to prepare an experimental design to verify scientific hypothesis, critically discuss results of the experiment, design practical experiment according to good laboratory practice.
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BI 311 MICROBIOLOGY
No cell phones during lab. Some labs require longer periods of incubation, but do not require you to check FB. Phones will be confiscated until lab is over, and reoccurring offenses will result in a deduction of points 5 pts off your final grade every. ... Intro to lab Techniques packet. week 2 Mon 1/30. Lab 3 a. Results – Enumeration b. Pure Culture (first streak) c. Smear Preparation d. Gram Stain. Lab 4 a. Pure culture (pick a colony) b. Spore Stain c. Acid-fast Stain. Techniques. week 3 Mon 2/06.
Abi biotechnology research internship
Shiguang Yu Yu’s lab provides summer interns with an opportunity to address the impact of health related issues (e.g. inflammation, autoimmune diseases) using a laboratory mouse model and cell culture. Interns will have the opportunity to learn immunology techniques, cell culture and flow cytometry and participate in the research about targeting inflammatory cytokines in immune cells. Hopefully this will increase the intern’s interests in biomedical research.
Pure culture techniques
Microbiology. Biol 275. Pure culture techniques. I. objectives. ... 2. Pigment Production: Pigments produced by bacteria are either cell bound (limited to cells and the colony), extracellular (pigment is released into ... Results of the Pure Culture Lab Exercise.
Research Assistant (Cowan Lab) | Harvard Stem Cell Institute...
Our lab combines cutting-edge genomic and proteomic techniques with animal and cell culture models. The Cowan lab is also a core part of the Harvard Stem Cell Institute and the Department of Stem Cell and Regenerative Biology at Harvard University. ... The research assistant will be expected to develop proficiency in molecular biology techniques, flow cytometry, histology, mammalian cell culture, and animal studies.
19 December
O’Neill Research Lab Introduction to Basic Techniques
Everyone working in my lab will be required to pass off tissue culture before they can proceed. Learning these techniques are essential as a contamination can be very costly in time, money, and effort. Each section must be passed off by instructors some sections will require you observing three different instructors to become proficient. ... Cell Freezing Cryopreservation of cultured cell lines is essential in the cancer research laboratory.
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BIT 566 Animal Cell Culture Techniques 2.
The interactions of proteins mediate numerous biological processes of cells. This course focuses on ways to identify and study protein-protein interactions, focusing on the advantages and limitations of each technique and how to apply the methods in a laboratory setting. In lab, students will perform a yeast two-hybrid experiment and a co-immunoprecipitation from proteins expressed in mammalian cell culture to confirm detected interactions.
9 July
College of Public Health
• Lab safety • Writing and reporting results (maintaining lab notebook, lab reports) • Experimental design, collection and statistical analysis of data, controls • Reading the research literature with understanding • Computer (data analysis, graphing, spreadsheet, literature search, databases) • Preparation of solutions • Pipetting liquids • Buffers and pH • General cell culture techniques, maintain cell cultures, viability and cytotoxicity assays. •
Technical and vocational programs | Lab Hours
CONCENTRATION OVERVIEW The Associate in Applied Science Biotechnology Degree is designed to meet the first year of general college requirements of a two-year technical program with the second year to be completed at Pitt Community College. ... CHM 131A Introduction to Chemistry Lab. CHM 132 Organic and Biochemistry. BIO 275 Microbiology. BTC 281 Bioprocess Techniques. BTC 285 Cell Culture.
Plant tissue culture techniques are also central to innovative areas of applied plant science, including plant biotechnology and agriculture. For example, select plants can be cloned and cultured as suspended cells from which plant products can be CELL AND TISSUE CULTURE ... CULTURE TECHNIQUES 2 Cell and Tissue culture LAB MANUAL Dr. Tarek Kapiel Using plant parts (explants) scientists now study the nutritional and regulator requirements for plant development and cell differentiation and for determining how plants respond to their environment at the molecular level, including how plant cells...
6 August
Cell Culture Facility | Biosciences Divisional Services
The Facility provides cell cultures for researchers to take to their labs for experiments, provides media and materials for checkout, and also allows use of laminar flow hoods, incubators and microscope for researchers who are using cells provided by the Facility, to use on site. ... A resource statement for grant applications is available below: The Cell Culture Facility provides mycoplasma testing, growing of cultured mammalian and insect cells and cell storage services. In addition, the Facility trains users in cell culture techniques and provides sterile hood facilities for users.
8 October
Two new mammalian cell culture feeds have arrived in the...
Students were asked to observe the cell culture they handled for contamination. Points were given to students for absence of contamination indicating proper aseptic techniques. ... They also learnt how to freeze and thaw cells as a separate lab. SLO 7: Cell culture contamination was covered during lecture and actual contaminated cultures were shown to students so that they can identify any contamination based on color change of media, turbidity and altered morphology of cells under the microscope.
Overview Brochure
Cell Culture & Tech Laboratory: outfitted to teach process development, optimiza-tion, and scale-up of different cell cultures. Analytical Instructional Laboratory: demonstrates in-process testing using modern analytical technology and techniques. 48-Workstation Wet Lab: equipped to teach a variety of scientific classes. Two Spacious Lecture/ Conference Halls: interactive distance learning environments serving 75 and 125.
Introduction to animal
In our lab: C2C12 immortalised skeletal muscle cell line Myoblasts were extracted from the thigh muscle of C3H mice 70 h after a. crush injury and cultured. ... Sensitivity to mechanical dispersal or enzymes; cell-cell contact may be required for proliferation Dispersed cells in culture are vulnerable Most primary cells require satisfactory adherence Some cells are not normally adherent in vivo and can be grown in liquid suspension In a mixed primary culture differences in growth rate may mean a loss of the cell type of interest – selection techniques Some.
F all s emester 2008 – biom 380
Module 1. Techniques in Cell Culture - Getting to know your cells. Week 9/1 Working with Cells in Culture: Analyzing Cell Adhesion and Growth Week 9/8 Stimulating Intracellular Activity: Isolation of Protein and Total RNA from Animal Cells. ... (No lab Week 10/13 a Mon-Tues are Reading Days). Allen (Smith). Module 4. Fundamentals in Digital Microscopy & Fluorescence Imaging - Seeing inside a cell. Week 10/20 Fundamentals of Microscopy, Cellular Imaging, and Analysis (Report due Week 11/3).
Advanced Molecular Biology Laboratory | Technical Support
Main Learning Outcomes. Students who successfully complete the course will be able to: • Perform the techniques essential for the operation of a molecular biology lab including those required for the manipulation and analysis of DNA, protein and tissue culture cells. • Understand the chemistry underlying the techniques used in a molecular biology lab. • Apply the techniques of molecular biology to the analysis of cell and protein function. • Apply the scientific method and understand what constitutes good experimental design. • Analyze and interpret data sets.
Course Content and Outcomes Guides | PCC
THEMES Cell growth requirements Behavior of cells in Culture. CONCEPTS Maintaining and assessing viability Contamination control Cryopreservation Laboratory organization Communication of procedures and results. SKILLS Aseptic Technique Observation Routine subculture Cell counting and viability Quality control Calculations Record keeping (notebook, lab records) Freezing and thawing cells Casual and formal oral communication Written communication Graphic interpretation of results Cooperation with colleagues Teamwork Time management.
9 December
TA Lab Assignments - Microtechniques, 2015
1/28 Wed Quiz; Laboratory Exercise #2 – Basic Manipulations of Cultured Cells. Demonstration of sterile technique & lab set up; plating of mdbk and 3T3-L1 cells. 1/30 Fri Refeed & Observe Cells. ... Lab Intro – Lab Exercise #2 Growth Curve Adipogenesis Transfections Organ culture IHC. TA. Lab Exercise #2 – Aliquot media; aliquot trypsin; set up lab; instruct students in proper cell culture techniques.
Animal cell culture techniques
Springer lab manual. Subject Term: Cell culture -- Laboratory manuals. Added Author ... In addition to basic techniques, a wide range of specialised practical protocols covering the following areas are included: cell proliferation and death, in-vitro models for cell differentiation, in-vitro models for toxicology and pharmacology, industrial application of animal cell culture, genetic manipulation and analysis of human and animal cells in culture.
theory and techniques of cell culture, preparing them for job...
Supplies: Any brand of bound notebook (non-­?removable pages), blue and/or black ink pens, permanent laboratory markers (any color(s), but must be water and alcohol resistant), lab coat. (optional). Course Description: The goal of this course is to give the student a solid foundation in the. ... 1. Propagate adherent and non-­?adherent cell lines. 2. Express exogenous genes in cultured cells by using a variety of techniques to transfer. DNA into cells. 3. Isolate cells from tissues to start primary cultures and propagate those cultures.
Instructor: Cheul H. Cho, PhD
Class handouts of lectures and lab protocols will be provided. Description: This laboratory course is designed to provide students with valuable hands-on experience in the field of cell and biomaterial engineering. Experiments include biomaterial fabrication and characterization, mechanical testing of biomaterials, colorimetric protein assay, the basics of cell culture techniques, cell-based assays, the basics of light and scanning electron microscopy, flow cytometry analysis, and image capture and analysis.
Practice Sterile Technique
Procedure/Practice. ** Read Tips for Sterile Technique and Cell Manipulation. ** ** Read Feeding Cells. ** · Do this exercise on a table in the teaching lab. · Work with a partner. Critique him or her. Communicate to your partner if you see her or him make a mistake or pull their hands to the “hand out of the hood” line. Good coaching at this stage will dramatically improve cell culture technique.
5 April
Claire T. Fournier
Biology, Immunology, Fall Instructor of advanced lab where students worked through cell culture activities and independent cell stimulation experiments. 2013. Graduate Student, Wesleyan University. Dept. of Biology, Thesis lab Mentored three undergraduate students and one master’s student in wet lab techniques. ... 2008-2011. Fournier 1. Dept. of Biology, Introductory Biology, Spring Teaching assistant for laboratory section. Worked with students on labs designed to study physiology, evolution, and behavior.
Jamaica Plain, MA 02130
Introduction to Cell and
Chapter 5: Standard Cell Culture Techniques Chapter 6: Looking at Cells Chapter 7: Contamination: How to Avoid It, Recognize It, and Get Rid of It Chapter 8: Serum-Free Culture Chapter 9: Primary Cultures Chapter 10: Establishing a Cell Line Chapter 11: Special Growth Conditions. ... Routine maintenance is essential to having a functional cell culture laboratory. While one or more persons may be designated to oversee this task, all users must participate to some extent. This section will deal with those tasks necessary to keep a cell culture lab in good working order.
SDSU College of Sciences
The page you were looking for does not exist, please make a selection from the menu on...
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15 December
Upstream Processing of Animal Cell Culture Products
The principles and techniques in culturing animal cells in vitro. Cell line characterization and quality assurance of a cell culture laboratory. The principles and techniques for establishing a seed culture from a frozen stock to a T-Flask to a bioreactor. Strategies for scale up of animal cells in culture for production of biologics. ... Companies looking to incorporate cross training into their employee base. Production managers, scientists in manufacturing, sales and marketing staff looking to understand more about these processes.
11 January
Biosafety Manual
BMBL describes four biosafety levels (BSLs), comprised of level-specific laboratory practices and techniques, safety equipment and laboratory facilities. Please contact EH&S at 212-998-1450 to determine laboratory Biosafety Level. ... chemical material; · Always wear protective equipment (lab coat, gloves, eye protection) appropriate for the task; · Change gloves frequently; · Remove gloves and lab coat and wash hands before leaving the laboratory; · Wash hands thoroughly after handling microorganisms, cell cultures, human blood or tissues and.
Growth and Maintenance of | Cells Sf9
Suspension Cultures: Suspension cultures should be passaged before they reach a density of 2.0 to 2.5 x 106 cells/ml and diluted back to 0.7 to 1.0 x 106 cells/ml. Example: A 100 ml spinner containing 50 ml of cell culture is at a density of 2 x 106 cells/ml. ... This subculturing method is very gentle and results in high cell viabilities. We typically use this method to dislodge adherent cell cultures. Definition: To dislodge cells from a surface by streaming medium over them. The protocol for this technique can be found in Adherent Cell Culture, page 13.
Slide Culture Technique for Fungi (Theory) : Microbiology...
. you are here->home->Biotechnology and Biomedical Engineering->Microbiology Virtual Lab II->Slide Culture Technique for Fungi. ... A mold or filamentous fungi consists of long, branched, threadlike filaments of cells called hyphae that form a mycelium, a tangled mass or tissue like aggregation. Vegetative hyphae grow on the surface of culture media. They form aerial hyphae, called reproductive hyphae, that bear asexual reproductive spores or conidia.
24 April
Los Rios Community College District
BIOT 312 Biotechnology Laboratory Methods - Microbial and Cell Culture Techniques 2 Units. Prerequisite: BIOL 300, BIOL 303, BIOL 310, BIOL 400, BIOL 440, BIOL 442, or BIOT 307 with a grade of "C" or better Course Transferable to CSU Hours: 18 hours LEC ; 54 hours LAB Description: This course covers basic concepts and techniques to work effectively in a bioscience laboratory. Topics include media preparation, microbial and plant cell culture techniques, biosafety guidelines, and DNA and enzyme diagnostic techniques.
17 August
Tulane University
Tulane University is actively seeking companies interested in commercializing a novel approach to generating neuronal cell cultures for laboratory use. Current approaches derive neural cell cultures from neuroblastomas in a two-dimensional culture environment. These cells do not truly resemble in vivo neuronal cells, leading to unreliable and sometimes unpredictable lab results, which costs valuable ... This technology employs state-of-the-art three-dimensional cell culturing techniques to produce neural cell cultures that are significantly improved over neural cell cultures currently in use for research.
Basic laboratory techniques in cell culture
Published: Atlanta : U.S. Dept. of Health and Human Services, Public Health Service, Centers for Disease Control, Bureau of Laboratories, Laboratory Training and Consultation Division, Virology Training Branch, [1981].
31 January
HVCC| College Catalog - Academic Programs - School of...
Biotechnology Certificate HEGIS #5604 Chairperson: Peter A. Schaefer Science Center, Suite 319, (518) 629-7453. The Biotechnology Certificate is designed to offer the advanced biology student specialized training in applied techniques of cell culture, immunology, analytical chemistry, molecular biology, biological imaging, and analytical cytology. ... Molecular Lab Techniques.
22 April
Immunology Lab | 8. Rules in the Tissue Culture Room
Lab math. In vivo versus In vitro studies. Clinical research. BioL 477. February 5, 2016 1. Introduction to Cell Culture Techniques (Watch Video from link below) The ability to culture cells outside the natural environment (i.e. from an animal or plant) has been named one of “medicine’s ten great discoveries”. ... His initial breakthrough occurred when he added the cells to fresh frog lymph and used a hanging drop technique for culture (see below). Primary Cell Cultures: Cells cultured directly from an animal or plant.
Abbott Bioresearch Center
Some lab experiences including aseptic technique, familiarity with cell culturing in flasks, wave bags and bioreactors, media/ buffer preparation, chromatography column operations and purification processing. Page 2. Abbott Bioresearch Center 100 Research Drive. Worcester, MA 01605. ... Daily tasks for this position involve some general lab culture techniques, such as aseptic manipulation, media preparation, bioreactor and shaker flasks operation.
Biotechnology < MiraCosta College
Students learn laboratory safety and documentation while acquiring skills in the maintenance and calibration of basic lab equipment, calculation and preparation of lab solutions and media, and routine handling of both bacterial and mammalian cell cultures (tissue culture). ... Instruction focuses on growth and manipulation techniques and long-term maintenance of various laboratory cell cultures that may include anchorage-dependent and suspension cell lines as well as stem cell cultures. BTEC 203: Techniques in DNA Amplification.
7 June
BITC 2431: Cell Culture Techniques This course discusses...
3 lec/3 lab. BITC 2411: Biotechnology Laboratory Instrumentation This course covers theory, application and operation of various biotechnology-related analytical instruments and addresses separation and identification techniques including electrophoresis, spectrophotometry and chromatography. ... Laboratory emphasis on the principles and practices of initiation, cultivation, maintenance, and preservation of cell lines are covered consistent with regulatory requirements, industry expectation, and Good Laboratory Practices (GLP).
3 February
T.C Istanbul Kultur Universitesi'nin ile ilgili bilgilerini sunar.\
In course content, cell and tissue culture techniques and basic principles, tissue culture room design, spesific devices and materials, safety rules in tissue culture laboratories, working conditions, contamination sources, sterilization and cleaning techniques, culture media and solutions, methods for cell culture, animal husbandary and care principles, primary cell culture methods, specific cell lines and their cultivation techniques, recent advances in molecular biology and.
21 October
Commonly refers to the culture of animal cells and
The history of cell culture dates back to early 20th century, when the first development of cell culture was to study, under the microscope , normal physiological events such as nerve development. Animal cell culture didn?t become routine laboratory technique untill the 1950s, where the need for cell culture, especially at large scales , became apparent with the need for viral vaccines when major epidemics of polio in the 1940s promoted a lot of efforts to develop an effective vaccine.
Beginning Molecular Biology Laboratory Manual
REFERENCES: R. Ian Freshney, Culture of Animal cells: A manual of basic techniques, Wiley-Liss, 1987. VI. ... Use only sterile pipets, disposable test tubes and autoclaved pipet tips for cell culture. All culture vessels, test tubes, pipet tip boxes, stocks of sterile eppendorfs, etc. should be opened only in the laminar flow hood. If something is opened elsewhere in the lab by accident, you can probably assume its contaminated.
31 July
Staying on Target: New Strategy Advances Stem Cell Culture...
In the embryotic stage of an organism’s growth, stem cells develop into specialized heart, lung, and skin cells, among others; in adults, they can act as repairmen, replacing cells that have been damaged by injury, disease, or simply by age. Given their enormous potential in future treatments against disease, the study and growth of stem cells in the lab is widespread and critical. But growing the cells in culture offers numerous challenges, including the constant need to replenish a culture medium to support the desired cell growth.
12 December
Core Laboratory: Cell Culture Workshop
Biological Core Lab. Internal Evaluation Services. Laboratory Management and Procurement. Research Operations and Building Services. ... Tuesday, March 21, 2017 | 4 p.m. - 5 p.m. Location. Science Center, Tapley Room 130. The Cell Culture workshop will provide a solid understanding of basic cell culture techniques through classroom and laboratory work.
21 March
PC12 Undergraduate Lab Protocol | III. Counting Cells
Schedule of lab activities for PC12 lab. In the weeks ahead there will be many events going on in this lab and it is important that you be up to par on technique and what is expected of you. Therefore, below is a generalized schedule of events that should keep you from missing any important dates and times for these experiments. WEEK 1: Cell Culture and PC12 Differentiation. 3/17 Meet in Cell Culture room at 1:00pm.
11 November
Cell Biology
Office Hours: Monday 1-3, Tuesday 1-2, Wednesday 1-3, Friday 11-12, by appointment, and by chance. If not in my office I’m usually in 132 (cell lab), 137 (across from the cell lab), or room 2. General Class Information The objectives of this course are to become familiar with cell structure and function, to understand some of the current experimental techniques and how to apply them, and to become familiar with some current literature. ... Lab # 3 Cell culture basics.
4 September
Antoniewicz Laboratory | Research Program
Research Interests. The Antoniewicz lab is developing next generation tools and techniques for analyzing, engineering and manipulating microbial and mammalian cells applied to specific problems in biotechnology (biofuels, pharmaceuticals) and medicine (cancer, obesity, diabetes). In our research we make use of modern techniques for cell culture, metabolic flux analysis, tandem mass spectrometry, molecular biology, bioinformatics and computational biology.
20 December
Cells on chips | Culture
standard tissue culture, and can be used to link cell culture with integrated analy-tical devices that can probe the biochemical processes that govern cell behaviour. Some cell-based microsystems simply represent miniaturized versions of conventional laboratory techniques, whereas others exploit the ... This typically involves higher cost, but has unique advantages for specialized applications, such as electrophoresis in glass devices. Much cell-based microsystem research takes place under a ‘lab-on-a-chip’ or ‘micro-total-analysis-system’ (µTAS) framework that seeks to create microsystems incorporating...
Winter Session 2016 MCB Professional Science...
MCB 5427-01 Laboratory Techniques in Functional Genomics Topic – Cell Culture Techniques Dates: January 8, January 9, and January 10, 2016; 9 a.m. to 5 p.m. 1 credit Enrollment Limit 6 Instructors: Charles Giardina and Michelle Rosado Room TLS 201 January 8, 2016: 9 a.m. to 5 p.m. January 9, 2016: 9 a.m. to 5 p.m. January. ... Prerequisite is MCB 5427 Introduction to Molecular Biology Techniques, relevant research lab experience, or permission of instructor. Participants learn the fundamentals of cell culture.
Culture Media | Special Culture Techniques
A culture medium is any material prepared for the growth of bacteria in a laboratory. Microbes that grow and multiply in or on a culture medium are known as a culture. ... Oxyrase is a bacterial respiratory enzyme that can be put in media - turns the petri dish into a mini-anaerobic chamber. Anaerobic chambers with air locks are filled with inert gas. Special Culture Techniques. Some parasitic and fastidious bacteria must be cultured in living animals or in cell cultures.
4 April
Cell Culture Lab - LECOM Education System
The mission of the Cell Culture Laboratory is to address fundamental questions about cellular physiology and biochemistry by using cell culture models for health and disease states. ... Using a cell culture model and a variety of molecular and imaging techniques, the goal of research in this area is to determine the role of the calcium-activated potassium channels in the differentiation of macrophages to osteoclasts.
19 July
Plant Physiology
Plant Organ Culture - Culturing Excised Tomato Roots. Objectives: Upon completion of this laboratory, you should be able to: use standard methods of sterile technique. surface sterilize plant tissues/organs. describe the uses for cell and tissue culture in plant physiological research. ... Pollen can be cultured by standard tissue culture techniques to form a callus. The callus can be induced to differentiate and a plantlet can be obtained. Note that the plants/cells formed are haploid.
14 September
DM Lab 05 | II. TECHNIQUES
Label all cultures with the name or number of the organism, and your name. Always clean the work area thoroughly with disinfectant before leaving the laboratory! The last step before leaving the lab is to wash your hands with Septisol. Gram stain technique. ... II. Techniques. The gram stain. Emulsify your sample taken from one colony with a sterile loop or needle in a very small drop of saline on a clean glass slide. Spread it out to leave a thin film. Allow smear to air dry. (Heating destroys the morphology of the cells.)
11 May
Biomaterials and Regenerative Therapeutics Laboratory
BE 302 Biomedical Engineering Laboratory (Spring). This course exposes to biomedical engineering lab techniques and analysis procedures. The lab provides hands-on experience with cell culture technology with emphasis on the principles and practices of initiation, cultivation, maintenance, preservation of cell lines and applications. Biochemical and biophysical characteristics of cells in culture. Analysis of cell viability, growth and proliferation. Basic training in microscopy, spectrophotometry, and immunological methods.
30 January
Lab 2: aseptic technique and transfer of...
After incubation, growth (development of many cells from a few cells) may be observed as one or a combination of three forms: a. Pellicle: A mass of organisms is floating on top of the broth (see Fig. 7A). ... After completing this lab, the student will be able to perform the following objectives: DISCUSSION. 1. Define the following terms: pure culture, sterile medium, inoculum, aseptic technique, and colony. 2. State and define the three types of growth that may be seen in a broth culture.
30 September
Academic Affairs | Course Catalog | ALS 421: Mammalian Cell...
This laboratory course provides extensive hands-on experience in KGI's lab-scale pilot plant for animal cell culture (part of the Amgen Bioprocessing Center). ... 1. The ability to culture cells using proper aseptic technique, using small-scale disposable cell culture vessels, such as T-flasks, shakers, and/or roller bottles. The ability to successfully freeze down cells for long-term storage, and later thaw with high viability.
9 August
Common Laboratory Techniques
The following general rules should be adhered to when working in the microbiology laboratory. 1. The inoculating loop is usually used for making transfers of bacterial cultures. The proper technique for flaming a loop with a Bunsen burner is described below. Allow the loop to cool sufficiently so ... A satisfactory smear will allow examination of the typical cellular arrangement and isolated cells. Note: make sure you resuspend the culture before removing a sample either by rotating or tapping the bottom of the tube and when removing a sample, do not flame the culture tube if it is plastic (the tube could melt).
26 February
Lab Module 4: Streaking for Isolation
Clark College. Kibota 1. Lab Module 4: Streaking for Isolation. Introduction In the1850s, Loius Pasteur developed the concept of the pure culture. This was an important intellectual step forward because the ability to work with pure cultures allowed Pasteur (and subsequent microbiologists) to study individual bacterial species. ... Use aseptic technique (Mod 2) to transfer cells from the stock culture to Sector 1 of the agar plate. Remember to cool the loop each time you flame it. a. Start from one end of the sector and move, in a zigzag path, towards the. other end. b. Be careful to not cross the sector line that you drew.
University of Hawaii at Manoa Catalog
MICR 431L Microbial Physiology Lab (2) (2 3-hr Lab) Components and metabolism of the bacterial cell; emphasis on techniques of analysis of metabolism and molecular structure. Co-requisite: 431. ... MICR 490L Virology Lab (2) (2 3-hr Lab) General laboratory techniques and related theories in virology; including isolation, cell culture, assay, purification, and identification of viruses.
15 December
SELCUK UNIVERSITESI
: Presentations and Seminars. : Course Internship. : Workshop / Laboratory Applications. : Field Studies.
10 December
Cell Culture Techniques | Marmara LLP | Laboratory
The aim of this course is to given information about cell cultures and their preparation techniques. ... Introduction of Cell Culture Cell Culture Laboratory Layout and Equipment Used in Cell Culture Types and Origins Cell Bank Systems Cell Bank System Applications Cell Freezing and Storage Methods Cell Viability and Toxicity Tests Tissue Engineering.
4 January
Cell Culture
Cell Expansion / Preparation of Cell Line Stocks: Cultured cells from your lab can be tested for mycoplasma, expanded, aliquoted, and cryopreserved in freezing media (5 x 106/vial). Preparation of cells for DNA or RNA extraction: Eukaryotic cells from the your lab or from ATCC can be expanded for nucleic acid isolation (1 x 10 8 cells). ... Cell Line Distribution. We are committed to assisting you with any cell culture questions such as contamination, slow-growing cell lines, techniques, lab design and lab maintenance.
13 June
Bi 1X, Spring 2013 | Session 1: Introduction on Lab Techniques
5. Copy the results into your lab notebook as best you can. Why does chlorophyll appear green? Spectrophotometry to measure optical density of a cell culture. It is often useful to determine how many cells one has in a culture—spectrophotometry is one technique than can be used to do this. ... 3. Insert your sample cuvettes into the spectrophotometer and record the results. References. 1. Bertani, L. E. “Cell Biology Laboratory Manual, 2003/04” Caltech. 2. Ausubel, F. M. et al, “Short Protocols in Molecular Biology” Wiley John & Sons Inc; 4 edition. (April 19, 1999).
University of Montana
Vero cell cultures infected with HSV at different multiplicity and at different culture conditions were studied. It was found that viral antigens could be detected as early as 1 hour after infection and cells infected for more than i; hours would be suitable for the IP study. Under culture conditions which decrease the content of viable virus, its presence in cell culture was detected by the IP technique but was negative by the isolation procedure.
Department of Biology | Cell Culture Lab
The core cell culture facility is a resource available to all biology faculty and student. It is located on the third floor of the Howell Science Building in room N302. It is a recently renovated laboratory that serves as a teaching as well as research facility. The facility is used to train both undergraduate and graduate students. ... In addition, we are teaching undergraduate workshops during the summer. These workshops are 2 weeks long and provide undergraduate students with necessary skills to work in a cell culture lab.
30 November
Alfred State - Courses by ID
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Links to the page contain: VETS 3024 Clinical Laboratory Techniques....
19 August
Department of | 14BT2002 biochemistry lab
and Crop Management Lab Developmental Biology Human Genetics and Genomics Vaccine Biotechnology Animal Biotechnology and Cell Culture Techniques Molecular Forensics Stem Cell Technology Human Physiology Cancer Biology Biopharmaceutical Technology Research Methodology Biochemical Engineering Metabolic Engineering Process Equipment Design Process Control Engineering.
Galter Health Sciences Library | Laboratory Tools and...
Laboratory Tools and Resources Owner: Pamela Shaw. Description: Websites and electronic resources that will help researchers find protocols and shared facilities Know of a good resource for this list? ... JoVE: Journal of Visualized Experiments This is an online journal showing hands-on video techniques for lab methods from the basic (cell culture) to more complex techniques.
26 August
Laboratory Techniques in Cell and Molecular Biology
A laboratory manual (from Professional Copy ‘n Print) is required. A recording notebook is required (3-ring preferred so you can keep track of printouts generated in class). A calculator is necessary for labs and quizzes. ... · Using a model for how one aspect of cell cycle is regulated, can you describe how your protein fits in with others (with which yours interacts and others) in order to accomplish cell-cycle regulation? Lab Practical Exam. You will be asked to demonstrate some of the techniques that you have learned during this class.
13 February
Stem Cell Core Facility: Cell Culture Facility
Sterile and aseptic cell culturing techniques will be practiced at all times. Only individuals who have been trained for cell culturing will be approved to use the sterile culture suites. ... Individuals who bake at home should not do so for 3 days prior to using the culture facilities. Lab coats, face masks, and booties must be worn at all times in the tissue culture rooms. All culture work in the Core must be done with reagents (media, growth factors, plasticware, etc) supplied by the Core.
Course Descriptions - Molecular & Biomedical Sciences...
BMB 441 — Immunology Laboratory A laboratory course to introduce students to diagnostic and experimental techniques routinely used in the immunology lab. Prerequisite or Corequisite: BMB 440 and BMB 471. ... BMB 471 — Cell Culture Laboratory A laboratory course devoted to eukaryotic cell culture techniques and applications. Prerequisite: BMB305. Lab 2, Cr 1.
1 December
Cell culture
The environmental virology/cell culture lab provides cell culture support for research conducted by Dr. Charles Gerba. ... We also provide training in all these areas to students, and research scientists from around the world involved in the environmental sciences. Patricia Gundy is a research specialist serving as the laboratory manager since January 2000. She received her Bachelors of Science degree in 1989 from the University of Arizona in Microbiology and Immunology.
3 February
NIST
All Associates will receive a stipend increase after one year of tenure. Additional Information About National Institute of Standards and Technology (NIST). Prior affiliation includes direct full-time employment relationships with the laboratory. Research contracts with universities that provide support for graduate students or faculty who perform research on campus are not ordinarily considered to be disqualifying.
28 January
Hands-on training on cell culture techniques...
Cell culture technology occupies a crucial place in all. A 2-DAY workshop on. Hands-on training on cell culture techniques and cytotoxicity assays. 17th-20th March, 2015. areas of biomedical sciences and healthcare research. ... The training will be for 2 days in 3 groups of 30 each. Each participant will be informed in advance of his/her group. Participants must bring their own clean lab coat which is mandatory.
Course Learning Outcomes of CELL CULTURE TECHNIQUES
2.3. Applies cell culture purification techniques. 2.4. Seeds isolated cells and applies viability tests. 2.5. Performs microscopic examination of cultured cells. ... Lectures with rich visual aids, lab practice, seldom home works. Students are expected to attend the lectures regularly, take notes and participate in discussions. Protocols for Neural Cell Culture Series: Springer Protocols Handbooks Doering, Laurie C. (Ed.)
cultures Sterile techniques Genetic manipulation of crop...
? Somaclones---Plants derived from any form of cell culture involving the use of somatic plant cells. ? Sterile--- (A) Without life. (B) Inability of an organism to produce functional gametes. (C) A culture that is free of viable microorganisms. ? Sterile Techniques---The practice of working with cultures in an environment free from microorganisms. ... Tissue culture using axillary buds and meristems. • From one to many propagules rapidly; • Multiplication in controlled lab conditions & Reduce stock plant space. • Precise crop production scheduling. • Long-term germplasm storage.
Chapter 1
As the name implies, the technique was elaborated first with undisaggregated fragments of tissue, and growth was restricted to the migration of cells from the tissue fragment, with occasional mitoses in the outgrowth. Since culture of cells from such primary explants of tissue dominated the field for more than 50 years, it is not surprising that the name "tissue culture" has stuck in spite of the fact that most of the explosive expansion in this area since the 1950s has utilized dispersed cell cultures.
Introduction to Cell Culture and the
• Self-sustained research lab with cell culture and nano-scale imaging capabilities. ... Cell Culture Facility. • Purpose: maintain experimental cell lines for nanoscale study and imaging. • Types of cells: mammalian cell lines, plant cell cultures, non-airborne pathogens. • Approved for BL-2 organisms, 2-18-09.
Detailed search of our online catalog
Technical Requirements. Contact Info.
22 April
Biotechnology (BTEC) | Cell Culture Techniques
BTEC 2030. Cell Culture Techniques. 2:0:6. Fall, Spring. * Prerequisite(s): BIOL 1615, MICR 2060 with. minimum grade of C- in each. Teaches basics of prokaryote and eukaryote cell culture; includes handling, storage, and maintenance of bacterial, mammalian and yeast stocks. Emphasizes media preparation and sterile techniques. Includes in vitro labeling and transfection. Course fee of $195 for lab applies.
Biotechnology | Health and Public Services
BTC-181 Basic Laboratory Techniques PREREQUISITES and COREQUISITES • NONE. This course introduces the basic skills and knowledge necessary in a biological or chemical laboratory. Emphasis is placed on good manufacturing practices, safety, sustainable lab practices, solution preparation, and equipment operation and maintenance following standard operating procedures. ... Topics include aseptic techniques, the growth environment, routine maintenance of cell cultures, specialized culture techniques, and various applications.
30 May
Policy | CICF | Cell Culture Lab
1 May
Molecular biology and genetics department
Lab 0. ECTS credit 6. Assessment methods and criteria. Course threshold Semester threshold. Activity Midterm exam Quiz Homework Semester project Laboratory Other Final exams. Quantity. 2 -1 ---1. 35 40 Percentage(%) 40 -----60. Course objective. ... Course contents Contents Introduction to Basic Mammalian Cell Culture Techniques Subculturing the cell culture monolayer forming trypsinization Passaged cells from suspension cultures Freezing the cells from monolayer cell culture Freezing the cells from suspension cultures Thaw in the cold environment and revitalization of human cells...
Biomedical Engineering
2. Professor Cynthia Reinhart-King projects: 1). Measuring Cellular Traction Stresses in Response to Dynamic Changes in Substrate Stiffness: Reinhart-King Lab. Statement of the Problem: Cardiovascular disease is the number one cause of death worldwide and atherosclerosis is recognized as the underlying pathology. ... 1. Learn aseptic technique, cell culture, hydrogel synthesis, and traction force microscopy. 2. Practice making hydrogel substrates and seeding cells onto them. 3. Determine the cross-linking exposure limits for endothelial cell viability using a live/dead assay.
Course Detail - Davidson College
Additional Links. Request Admission Info. Schedule a Visit. How to Apply.
26 June
Course Directory : University of Vermont
Skip to main navigation Skip to local navigation Skip to main content.
28 May
Neuroscience Department - Zou Lab Projects
Experimental Approaches: Our lab is trying to reconstruct the activation cascade of transcription factors and their downstream targets that controls the developing axon growth and rekindles the axon regenerative ability of adult neurons. The goal requires the integration of molecular biology, cell cultures based assays, biochemical techniques, imaging, mouse genetics and in vivo mouse models of axon and neuronal injury.
2 December
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3 April
AUB - Biology - Research Labs and Cell Culture Facilities
The Department includes 3 common research laboratories located on the first, second and third floor. These include different research equipment such as deep freezers-LN2-ELISA readers/washers-concentrator-electroporator-V/visible spec-cell harvester/counter-sonic dismembrator-anaerobic chamber-orbital incubator shaker-high speed centrifuges-Gel doc(next year)-Gradient cyclers. ... Marine lab. Cell culture rooms.
30 December
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31 July
Bioengineering | Electrophysiology Lab (204 Rhodes)
Electrophysiology Lab (204 Rhodes). The Electrophysiology Laboratory is equipped for patch clamp analysis, and is associated with. the cell culture laboratory in 206 Rhodes which prepares living cells for physiology tests. ... Histology and Advanced Imaging: Dr. Terri Bruce, Co?Director; Linda Jenkins, Chad McMahan, Dr. Bruce Gao Stem Cell Biology: Dr. Carol Brenner, Co?Director; Dr. Dan Simionescu Cell, tissue, and molecular biology techniques are the cornerstone of biomedical research and regenerative medicine endeavors.
Pages · Towson University
11 June
iPS Cells Culture Class | School of Medicine | University of...
Stem Cell Lab Tour 2015. ... The Core offers an iPSC culture class upon request (3 participants required). This one week course is a hands-on class that focuses on techniques required to maintain, preserve and amplify iPSC. Topics include: Passage of mouse and human iPSC; Methods to maintain iPSC in a pluripotent state; Cryo-preservation and thawing of iPSC and transitioning of iPSC from feeder cells to feeder-free cell culture systems.
24 August
Biotechnology
In addition, students seeking the AAS degree complete six WECM approved biotechnology (BITC) courses: Introduction to Biotechnology, Biotechnology Lab Instrumentation, Biotechnology Laboratory Methods and Techniques, Cell Culture Techniques, Molecular Biology Techniques, and a Biotechnology internship.
The effects of aseptic and sterile technique
Along with sterile techniques that all labs utilize, researchers also use aseptic techniques when studying bacteria and transferring a target species of bacteria to cultures. Major sources of contamination include airborne contaminants and the use of non-sterile equipment (Ryan, 2002). ... 2002. Understanding and Managing Cell Culture Contamination. Acton, MA: J. Ryan. Fierer, N., M. Hamady, C. Lauber, and R. Knight.
Pumps for microuidic cell culture
A clear concern for performing cell culture in microuidic-based devices is deciding on a technique to deliver and pump media to cells that are encased in a mi-crouidic device. In this review, we summarize recent advances in pumping techniques for microuidic cell culture and discuss their advantages and possible drawbacks. ... Several applications have been re-ported in which cells were cultured on a lab-on-a-disc plat-form [10, 56–58].
Biology 342 - Microbiology | Lab Notes/Items due exercise
Introduction Lab safety Microscopy Aseptic technique Staining Microbial ubiquity Identification of bacteria Pure culture techniques Bacterial population counts Microbial growth curves. Lab Notes/Items due exercise. Quiz 1. ... When studying bacteria, it is important that the bacterial culture be pure, meaning that it contains only cells of that bacterial species. Otherwise, the results of any tests or procedures are meaningless. From a clinical standpoint, it is imperative to avoid cross-contamination of samples, lest the wrong diagnosis be made.
Drug Design and Discovery | Masters in Biotechnology Program
Credits: 3; Semester: Fall or Spring; Prerequisites: None. This is a comprehensive hands-on, laboratory-based course that introduces students to core techniques such as electrophoretic analysis of nucleic acids and proteins, polymerase chain reaction (PCR), Southern and Western blotting, ELISA, protein purification, tissue culture, DNA cloning, cell culture and Bioinformatics.
2 February
Science
BIOL 1402L - Cell Biology and Genetics(Lab) Credits: 1.00 This laboratory investigates principles and applications of cell biology. ... Perimeter College Division: Division of Science. BIOL 1913 - Microbiology Credits: 3.00 This is a study of fundamental principles including basic culture and staining techniques, cellular metabolism, sterilization, disinfection, basic principles of immunology, and etiology of some infectious diseases.
13 December
U of M: Department of Mechanical Engineering: IEM Cancer...
...cell lines, and establishing protocols for both hindlimb and dorsal skin fold (intravital window) tumor propagation and controlled substance use in their own labs. ... 4. Cancer Cell Culture. ... a) Sterile cell culture techniques b) Preparing cell growth medium c)...
6 December
Tissue Engineering A Blood Vessel Mimic While
Higher growth trends were found on surfaces outside the cell culture lab, in the general TE lab area. These findings were used to provide overall suggestions on tracking microbes for long-term durations in ongoing BVM setups to directly improve the overall sterility assurance of the studies. As the project reached its conclusion a look back at all the BVM setups and contamination assessments lead to a few suggestions for improving aseptic techniques within the TE lab, such as monitoring microbial growth in the culture processes, creating limit specifications...
Fatal error: 'break' not in the 'loop' or 'switch' context in...
Links to the page contain: Cell Culture Techniques....
14 August
Biotechnology Laboratory Sciences
• Biotechnology Technician • DNA Finger printer • Cell Culture Technician • Quality Control Technician • Research Assistant • Bio-Manufacturing Assistant • Laboratory Technician. ... • Students will be able to perform techniques relevant to biotechnology. • Students will understand the biochemical properties of DNA, RNA and describe the PCR steps. • Students will demonstrate an understanding of the importance of following lab safety and proper lab documentation.
Gaziantep University
Directorate of health, culture and sports. Office of strategy development. Directorate of construction and technical works. Office of press, printing, and public relations.
2 July
Advanced Microscopy Techniques laboratory | Sabanc?...
Advanced Microscopy Techniques laboratory. Objectives and specifications. Retrovirus and cell culture incubator for human and animal cells. Confocal microscope for the measurement of living cells in five various wavelengths and for studying the fluorescent molecules inside biological cells. FRET technique for measuring the interaction of molecules within a cell, and observing the inter-cell organelles on a nanometer scale.
14 June
Lectures, Practical Courses, Presentation, Seminar, Project, Laboratory Applications (if necessary). ECTS / Table Of Workload (Number of ECTS credits allocated). ... 0. 0. Lab Study.
12 August
Course Description
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Links to the page contain: Cell....
10 April
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Links to the page contain: Lab: molecular cell biology....
4 May
SAMPLE | Lab Techniques
Lab Techniques: Three-dimensional Cell Culture and Cell Line maintenance, Gel Electrophoresis (Zymmography), Western Blot, Elisas, Flow Cyometry, Immunoflourescence Staining, and RNA extraction. PUBLICATION. Ferrera, V.P., Cohen, J.K., Wallace, C.R. (2007) A dissociation between spatial attention and motor response selection in prefrontal cortex of.
ASEPTIC TECHNIQUES | Button your lab coat.
DEFINITION. Aseptic techniques is defined as a method that keeps undesirable microbes from contaminating a pure culture. Only a single species of an organism is what one should work with in the laboratory. It is important that other microbes do not contaminate the broth the obtained "pure culture" or when trying to isolate this pure culture. ... *No food or drink in the lab. *No dangling jewelry. *No open-toed shoes. *If your hair is long, put it up. *Button your lab coat. *No cell phones. *Wear Gloves.
13 June
Courses :: Andrews University
BIOL 430 (F) Molecular Laboratory Techniques Acquaints students with modern lab techniques of molecular biology. The manipulation and study of nucleic acids and proteins using model systems. ... Taught by: Dr. Smith. BIOL 447 (S) Cell and Tissue Culture A study of the methods and applications of animal cell culture. Topics will include laboratory safety and equipment, primary and established cell lines, culture contamination, cell transformation and differentiation, and cell transfection.
1 April
LAB 2: Staining and Streaking
LAB 2: Staining and Streaking. Protocols for Simple stain, Gram Stain, Streak Plate Technique and Culture Maintenance. ... Thus, there is an affinity between a positively charged color ion and the negatively charged bacterial cell. In the Direct or Positive Staining Procedure a cell takes up a positively charged dye and becomes stained. Methylene blue, crystal violet, and safranin, are all basic dyes.
Exercise 4: Pure Culture Technique – Preparing a Streak Plate
Each single colony consists of a cluster of cells that originate by cell division from a single bacterial cell. Thus each isolated colony represents a pure culture of bacteria. To do this, you must first learn aseptic technique. This is a special set of procedures designed to prevent contamination. ... 4. Label the agar-side of the plate with your initials/seat # and ‘Mix’ to indicate the bacterial culture used. Incubate plate agar-side up in the appropriate incubation box until next lab period.
altweb:mabs:ardf:hendriksen | General Cell Culture Supplies
In perfusion culture systems (e.g., hollow fiber bioreactors), cells are maintained in a compartment separated from the media reservoir by a selective membrane. Cells are perfused continuously with media, but are not exposed to shear. As perfusion systems are more effective for supplying nutrients and removing waste, high-density cultures approaching 107-108 cells/ml are obtainable. The way media is processed characterizes cell culture techniques15.
24 June
§ Cell Culture: Pipetting, centrifuging, aseptic technique...
fixing, gram staining, acid-fast staining § Animal Handling: General care of mice & rat lab models; subcutaneous, intraperitoneal & intravenous injections; general. anatomy & dissections; cardiac punctures for blood collection § Analytical Techniques: Bradford-Lowery Protein Assays, SDS-PAGE Gel Electrophoresis, Titrations, Column. ... 2012 – July 2012. Research & Development Laboratory Assistant. § Assisted research scientists by maintaining cultures of several prostate cancer cell lines for assays and animal injections.
Scheduling Information, Biotechnology - Cell and Molecular...
The Scientific Communications & Lab Techniques courses are very strongly recommended & are expected for Graduate Assistantship eligibility. If you have a significant amount of prior lab experience, talk with your advisor before registering. ... (Total: 9 Credits). Winter Year 1 CMB 620 Cell & Tissue Culture 2 Th 6-9:50, 543 CHS. CMB 506 Advanced Molecular Biology 3 W 6-8:50, 710 EC. CIS 661 Intro to Medical & Bioinformatics 3 T 6-8:50, EC.
20 February
M.S. in Biomedical Sciences | LIU
Topics selected for study include sterile techniques, cell nutrition, media preparation, establishment and maintenance of callus and suspension cultures, growth measurement, morphogenesis, cell isolation, tissue and organ culture. ... In-depth coverage of concepts of cell origin and differentiation, as well as the molecular concepts of disease and current trends in research are covered. Quality control experience in lab practice, marrow differential counts, histochemical and biochemical techniques are included in-depth.
10 October
Biotechnology Courses - Ivy Tech Community College...
Lab safety, documentation, units of measurement, instrumentation lab solutions, aseptic technique, establishing a pure culture, plasmid DNA isolation, restriction enzyme digestion, DNA agarose gel electrophoresis, PCR, DNA ligation and transformation of bacterial cells will be introduced during the course. ... BIOT 201 Cell Culture and Cellular Processes (4 credits) Prerequisites:BIOT 101 and CHEM 105 or CHEM 111 or Program Advisor Approval.
15 April
UCCS Home - University of Colorado Colorado Springs
More than 300 days of sunshine. The Colorado altitude and proximity to the mountains makes for a great climate, year-round. Colorado Springs. Find out why Colorado Springs is ranked one of the Top 5 places to live in the U.S. REACH HIGHER. CHALLENGE ...
31 July
Biology 382 – Techniques in Molecular Biology – Syllabus...
Lab: safety, basic techniques: pipetting, sterile technique, bacterial culture. (Next day: check plates and tubes). Discuss research project selection. ... (Evening before) Set up overnight culture of DH5?. Lab: Mid-log suspension culture, classic competent cells preparation. Calculations sheet and graph due at end of lab. Lecture: Questions and hypotheses using molecular biology.
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Links to the page contain: National Policy On The Transfer Of Scientific, Technical And......
15 December
Cell Biology
Methods. Prokaryotic Cells Prepare a wet mount of bacterial culture 1. Take a culture tube containing bacteria and re-suspend the cells by rolling the tube between your. palms. The solution should look turbid. ... Cell biology lab sheet Prokaryotic and eukaryotic cells. Names: _ Always, choose a few, ideal examples and draw them accurately – don’t draw thousands of cells that all look alike! Prokaryotic Cells Record your observations of the bacterial culture.
Courses - Bioscience (Microscopy, Genomics...)
Description: Guided lab project using widefield fluorescence and/or confocal microscopy plus cell culture, IHC and digital imaging: Image acquisition, processing and analysis. ... Course Number: BIOSC 7 Practical Mammalian Cell Culture Units: 1 Class: .5 hours lecture, 1.5 hours laboratory (GR or P/NP) Co-requisite: Biosc 2 Acceptable for credit: CSU Description: Introduction to the basics of mammalian cell culture: Sterile technique, media preparation, and cell counting; cell lines, including adherent and non- adherent cells; response to infections.
18 March
SECTION 13 | Catalog
ANTH 102 CULTURAL ANTHROPOLOGY (3) Three hours lecture per week The study of recent and modern societies using a cross-cultural perspective to gain an understanding on the range of human expression in culture and society. ... BIOL 404 PLANT AND ANIMAL TISSUE CULTURE (3) Two hours lecture and three hours lab per week Introductory course in the theory and concepts of animal and plant cell and tissue culturing including the fundamentals of tissue culture techniques, subculturing and maintenance of cell lines and stem cell technology.
Lab 9
For transformed cells to grow in the presence of ampicillin you must: 1. Provide them with nutrients and a short incubation period to begin expressing their newly acquired genes. Read the lab exercise and follow the directions carefully. You will do this lab in lab groups of 3-4 students. ... You will practice using sterile technique, as instructed at the beginning of lab session, before you do the experiment. When culturing bacteria, you must not introduce other, contaminating bacteria into your culture.
LAB 3: Basic Microscopy and Cytology
The purpose of this series of exercises is to introduce you to the correct use of the compound microscope, the standard techniques to differentially stain and observe living cellular materials and to learn the structure of cells. 3.1. Putman’s Biol 160 Lab 3: Basic Microscopy and Cytology. ... The purpose of this exercise is to allow you to explore the one-celled, microscopic world of living cultures! Materials and Methods. Your instructor will have dishes of living cultures at the demo table. Using the pipette provided, obtain a drop of culture from near the bottom of the culture dish, or from near any solid objects in the...
Cell Analysis and Separation
While large-scale animal cell culture has become quite common, a number of important questions/issues remain unresolved. For example, what is the optimum hydrodynamic environment for a suspended or anchorage- dependent cell? What is the mechanism of action, from a molecular point of view, of surface active agents in cell culture medium? ... Chalmers Lab for Cell Analysis and Separation.
9 August
Doing good science: authenticating cell line identity
While the development of better sterile techniques helped decrease cross-contamination, few tests were available to determine which cells were already affected. Since then, however, standardized methods have been developed that are both quick and inexpensive to perform. ... However, cross-contamination continues to be a problem. With the increasing number of new cell lines and the high rate of cell culture use in labs worldwide, significant gaps have been created in basic principles of quality control (i.e., cell line authentication).
Biotechnology Co-Listed Courses
Topics include: an introduction to fundamental methods used to grow animal cells in culture; associated principles of cell structure and function; aseptic technique; preparation and use of various culture media; cell counting and dilution; maintenance and propagation of cell lines; origin and uses ... Upon completion of this course, the students should be able to: • Complete calculations that are routinely encountered in a protein lab such as buffer preparation, dilutions, protein concentrations and standard curves, enzyme activity, and specific activities • Complete laboratory techniques that are commonly used in protein...
13 December
Solving a 48-year-old mystery: scientists succeed at growing...
Human noroviruses – the leading viral cause of acute diarrhea around the world – have been difficult to study because scientists had not found a way to grow them in the lab. Now, more than 40 years after Dr. Albert Kapikian identified human noroviruses as a cause of severe diarrhea, scientists at Baylor College of Medicine have, for the first time, succeeded at growing noroviruses in laboratory cultures of human intestinal epithelial cells.
26 August
UCSD - The Ghosh Lab - Projects | Dissociated cells
Ghosh Lab Protocols: Slice overlay assay. ... Sections are collected using a droper (large opening flamed-tip Pasteur pipette) and platted onto a membrane insert of a 6-wells plate (Becton Dickinson-1 micron pore size). Put 1.8 mls of slice culture medium beneath the insert about 30 minutes before platting and leave the plate in a cell culture incubator (37°C-5 percent CO2).
14 February
BIO 101 General Biology I
Practice of fundamental staining techniques employed to study cellular and sub cellular structures, The techniques include; cytochemical methods, sub-cellular fractionation, cell cultures, karyotyping and permanent slide preparation. Prerequisite: BIO 203. BIO 252 Physiology (3-0)3. ... BIO 355 Microbiology Lab. Laboratory course for BIO 303. BIO 356 Plant Biology (3-2)4.
27 December
BIO 315: Advanced Molecular Biology and Biochemistry Lab...
A laboratory course for junior-/senior-level students interested in research methods in molecular biology and biochemistry. Experimental topics include enzyme kinetics; protein isolation, purification and analysis by electrophoresis and immunofluorescence; cell culture techniques; and advanced molecular genetics. Three hours lab. (0.5 credit.) Prerequisite: BIO 310, CHM 310 or concurrent registration in BIO 310 or CHM 310.
29 August
Geetha Durairaj, Kaiser Lab 2015
Transfer poly-­?lysine solution to storage container and return to 4°C. • Aspirate excess solution from 50 ml centrifuge tube containing coated coverslips • Wash coverslips twice with water • Remove coverslips one-­?by-­?one and put on filter paper to dry inside cell culture hood) • Once dry, UV sterilize for 20 minutes and store coverslips in 50 ml centrifuge tube or cell culture.
Obsolete URL
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13 December
Educational
3 periods (3 lec.) Technical, sociocultural, and political information on environmental science and technology for non-environmental majors. Includes ecosystems, population impacts, hydrological systems, air pollution, and environmental toxins. ... 6 periods (3 lec., 3 lab) Principles and methodologies of protein expression, isolation, identification and purification. Includes immunological and cell culture techniques. Information: Consent of instructor required before enrolling in this course. Offered: Spring.
BTEC | Catalog
BTEC 2050 BIOTECH LAB METHODS AND TECHNIQUES (60-30-5) Prerequisites: CHEM 1211, CHEM 1211L or CHEM 1212, CHEM 1212L Co-requisite or prerequisite: BTEC 2010 Introduces the basic skills and knowledge required in biological and chemical laboratories. Emphasis is placed on safety, documentation, measurement, spectrophotometry, polymerase chain reaction and laboratory solutions. BTEC 2100 CELL CULTURE (30-60-4) Prerequisite: BIOL 2117, BIOL 2117L Covers the culturing and maintenance of various cell types.
3 December
Cell Culture Lab
The Cell Culture facilities provide various resources for the isolation and culture of animal and human cells. Previous studies have successfully isolated and cultured cells from various musculoskeletal tissues, including bone marrow, periosteum, adipose tissue, tendon and cartilage. The cultured cells are used for upstream research work (molecular and genetic studies) or downstream studies such as in-vivo studies using animals and clinical trials. The Cell Culture Lab is equipped with
2 December
BSC 354 - Biotechnology Laboratory II:Cell... - Illinois State
Applications and theory of cell biology to study eukaryotic systems using biochemistry, cell culture, and immunology techniques. Lecture and lab. Materials charge optional.
30 September
Bio314: Advanced Cell | Grow cell cultures Materials
techniques or procedures could be done to confirm that it is the GFP that is glowing? ... You will use a UV light throughout this lab. Create a data table that will contain a list of ... Grow cell cultures Materials. 2 - Transformation plates from kit 1 (LB/amp/ara & LB/amp).
Research me this
Laboratory Techniques obtained from Classwork. • Sterile Technique. • Recombinant. • Fluorescence. • Media Preparation • Cell Culture Plating • Gel Electrophoresis • PCR. ... Colorado State University, Fort Collins, CO. July 2011-Present. Research Assistant in the Krapf Research Lab. • Organization of research data files on shared networks and external hardware devices. • Designing and building an enclosure for the laboratory infrared laser system.
Slide 1
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1 October
Laboratory techniques: Grading, Cell Suspensions
Laboratory techniques: reagents, reaction grading; cell washing; sample collection. Blood banking reagents. The techniques used in Blood Bank involves mixing antigens, usually on red blood cells with antibodies. ... In almost all blood bank techniques we have red cells with antigens present. These red cells may either reagent red cells with known antigens, patient red cells, or donor red cells. The reagent red cells are commercially prepared and have all the red cell antigens identified.
20 October
Professional & Technical - Marine Biotechnology - Brunswick...
We also have a cell culture lab which includes many different species of algae that are used as a food source for the invertebrates as well as many other applications from biofuel to natural food supplements. Coursework includes; basic lab techniques, genetics, recombinant DNA technology, cell culture, algae culture, invertebrate culture, fish genetics and breeding, marine biotechnology, and lab experience or aquaculture project.
12 January
BIO 241
Bio 241 cell biology lab manual. INSTRUCTOR: Nesrin Ozoren LAB ASSISTANTS: Sibel Ugur. Tolga Aslan Tuncay Seker Cigdem Atay. Bogazici University Department of Molecular Biology and Genetics. ... Since its establishment, animal cell culture techniques have allowed scientists to solve many puzzles on animal cell biology by allowing them to work under very strictly defined and controlled conditions.
Science Laboratory Technology, A.A.S
BTEC?286 Cell Culture Techniques Lab 0?9?3. Advanced course in a cell culture. Introduces techniques such stem cell culturing, transfection, cellular cloning, and biomarker identification. Students expand upon techniques mastered in BIOL-266. This class is intended for students in the Biotechnology concentration of the Science Laboratory Technology degree program.
Demo: Cell Culture Stem Cells Lab 1: Part 1, Cell Culture
Students will learn the techniques and equipment of bench research including cell culture, immunofluorescent imaging, soft lithography, variable stiffness substrates, application/measurement of forces and other methods. Students will integrate classroom lectures and lab skills by applying the scientific method to develop a unique project while working in a team environment, keeping a detailed lab notebook and meeting mandated milestones.
Hampton University : Human Resources : Employment...
Standard laboratory techniques required for this position include mammalian cell culture, molecular biology and cell biology. Competence and independence in conducting experiments is expected. Good communications skills and the ability to work within a group is also required. The successful candidate will be a highly responsible individual and will be required to maintain the lab and aquarium equipment.
17 February
LABORATORY FACILITIES | 7. Plant Tissue Culture Lab
CELL BIOLOGY AND MICROBIOLOGY LAB This Laboratory equipped with facilities such as Aseptic culture room, washing and dressing rooms. ... GENETIC ENGINEERING AND MOLECULAR BIOLOGY LAB Molecular biology and Genetic Engineering techniques are important tools for molecular studies, gene transformation for production of genetically modified products developments and gene characterization studies etc.
The Cervical Cancer Research Lab of Dr. T.-C. Wu
Cell Culture +. ... Intracellular cytokine staining in this lab is perfoemd using the Cytofix/Cytoperm kit according to the manufacturer's protocol BD PharMingen. ... This technique allows for the direct enumeration of antigen-specific T cells by their receptor...
14 July
Microbiology 7
Cell Biology Laboratory Manual Table of Contents. Techniques Manual Table of Contents. Lab Safety Sheet. Related Topics. ... We must have pure cultures for doing work in broth in order to know which bacteria are responsible for the effects we observe.
22 January
Cell Culture Lab
Click here for information about the Equipment of Cell Culture Lab. ... Technical Operations Officer.
26 May
Aseptic Technique and Good Cell Culture Practice To...
5 Discard gloves after handling contaminated cultures and at the end of all cell culture procedures. 6 Equipment in the cabinet or that which will be taken into the cabinet during cell culture procedures (media bottles, pipette tip boxes, pipette aids) should be wiped with tissue soaked with 70% ethanol prior to use. ... Ensure you add enough complete media to stop the action of trypsin and work to avoid wasting media and plastics, but ensure aseptic techniques are maintained.
Core Facilities — BioFrontiers | Cell Culture Facility
This lab houses a Phoenix dropsetter for crystallization, RockImager system for crystal imaging and two Rigaku R-axis IV++ image plate systems. Cell Culture Facility. ... It also has two automated liquid nitrogen storage units and an inverted microscope with both light and fluorescence sources. The facility is supervised by Theresa Nahreini, who trains all users in sterile techniques and oversees daily operations of media preparation, mycoplasma testing and maintenance of cell lines.
15 May
Expected May 2009
Lab Techniques ... Function Generator, Digital Multimeter, Spectrometer, Fluorescent Microscope with CCD technology, and Scanning Electron Microscope Three-dimensional Cell Culture and Cell line maintenance, Gel Electrophoresis (Zymmography), Western Blot, Elisas, Flow Cytometry, Immunoflourescence Staining and RNA extraction.
Why are we determining the frequency of antibiotic-resistant...
This is accomplished indirectly by spreading the cells on the surface of an agar plate and then counting the number of visible colonies after the plate is incubated. The inherent assumption of the technique is that each colony on the plate consists of millions of cells that arose, during incubation, from a single cell in the original sample. ... 2.7. Lab 2. Serial dilution and plating of a bacterial culture. 4/11/06. Your instructor may ask you to enter your data in an EXCEL spreadsheet . You will need to provide the following information: • total cell concentration in the original culture (cells/ml) • concentration of RIFR mutants...
Laboratory technical staff
· Perform routine cell biology techniques (sterile tissue culture of cell lines or primary cells of insect and mammals; stable cell line creation; histo- or cyto-chemistry; adenovirus or lentivirus production, purification, and transduction; cell transfection and fixation; microscopy; cell-based assays; etc.). ... · Knowledge on proper operation of certain lab equipment (plate reader; gel imaging system; scanner; centrifuges; autoclave; shaker incubator; upright and inverted microscopes; etc.) and performing preventive maintenance of laboratory instruments. · Familiarity with computer and software packages.
Regulations scheme and syllabus
UNIT II -Bacterial cell- structure and functions of cellular components-cell wall composition of Gram positive and Gram negative bacteria, sub-cellular organizations, flagella, capsule and spores; Bacterial Staining; antimicrobial agents-antibiotics, and antibacterial agents and their mode of action; antibiotic resistance. ... UNIT-I History of Tissue Culture technique. Requirements for a Cell Tissue Culture lab like Laminar Air Flow device, sterilisation scheme for culture chamber. Totipotency of plant cells- dedifferentiation and dedifferentiation.
The New School | Course Catalog
The New School in New York City offers degrees in liberal arts, music, design, social sciences, as well as certificate programs...
7 March
Btc 181 basic lab techniques
Upon completion, students should have an understanding of the theory, practice, and application of recombinant DNA techniques. There is a $11.25 lab fee for this course. ... Btc 285 cell culture. Prerequisites: Take One: BIO 175 or BIO 275 Corequisites: None Local Prereq: Take BTC 181 with a grade of C or better This course covers principles of microbiology and the impact microorganisms have on man and the environment in industrial settings where controlled environments are commonplace.
28 April
Scientific Calculations and Basic Lab Techniques
BIO354: Cell Biology Laboratory. Laboratory 1 Scientific Calculations and Basic Lab Techniques. ... Suppose that you treat a culture of Chlamydomonas acetic acid to lower the pH and then after 30 seconds, restore the pH to about 7.0 with a dilute solution of KOH. You place the deflagellated protists in a flask in a nutrient medium and then remove samples every 10 minutes for a total of 60 minutes.
Chemistry Lab Techniques HomePage
Soon to be the home of the Chemistry Lab Techniques Home Page.
10 April
Infectious Haematopoietic Necrosis Virus - Induced Cell Death...
A series of approaches to detect cell death were conducted in different cultured cells using TUNEL (Wutzler et al. 2001) and Annexin V or both techniques (B e l l et al. ... Preparing cell cultures and in virus infection. The epithelioma papulosum cyprini (EPC) cell line (286 passage) was kindly supplied by dr. G. Bovo from the. Instituto Zooprofilatico Sperimentale, Padova, Italy and propagated routinely at 25 °C (Fijan et al.
Exercise 10: Pure Culture Technique : Check results
2 Anaerobic Agar plates (all 3 on one, line inoculations). label 1 ANAEROBIC (put in jar), 1 AEROBIC (put in bin). Exercise 9: Aseptic Technique: Check results Broth to broth, Slant to slant, Plate to slant. ... Figure 18.4. 1. SCCC BIO244 Lab 6 Notes. Obligate aerobes: 20% O2 Obligate anaerobes: O2 toxic Facultative: w/ or w/o, better with O2 Microaerophiles: 5-10% O2 Aerotolerant: ignore O2. Fig 27.1.
Chapter 12: Cell Cultures - Introduction
Typically, prokaryotes are easier to culture in a laboratory because most of them have less stringent nutrient requirements. For this lab we will utilize bacterial cultures grown in nutrient agar, an environment in which human pathogenic organisms are extremely unlikely to grow. ... Each day students will observe these transfer cultures with an inverted phase contrast microscope, and remove aliquots for cell counting with a hemacytometer. Simultaneously, they will check on the viability of the cells through a dye exclusion technique.
9 April
Union County College
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21 May
Midwestern University-Biomedical Sciences
These studies utilize estrogen agonists/antagonists, cellular adhesion molecules and all of their corresponding roles during bone differentiation. Techniques: Molecular Biology and Biochemical Techniques listed below are utilized in our lab. Cell culture RT-PCR, PCR, Western Blotting, Immunoprecipitation Immunohistochemistry Immunofluorescence Enzyme Assays Southern Blotting Northern Blotting RNase Protection Assay Plasmid Preparation and Purification.
Services - Salk Institute for Biological Studies
All new, approved cell lines will be cultured under quarantine conditions until they have tested clean for mycoplasma in 2 consecutive tests. 2) All new STEM Core users must be approved by, and go through a brief orientation with the Core Director, or Lab Manager. ... The Core Director and staff can assist new researchers with the process of filling ESCRO protocols. For training purposes, new investigators can begin working under the existing stem cell culture techniques training protocol.
18 November
Applied Biology Cell & Tissue Culture Lab
We are located on 180-acres in a 35,000 square-foot facility in a Certified Technology Park, on the south campus of Rose-Hulman in Terre Haute, Indiana. Applied Biology Cell & Tissue Culture Lab. ... Mechanical testing in the laboratory is conducted utilizing a state-of-the-art biaxial materials testing machine along with strain gage and digital image correlation optical techniques.
12 March
Lab Research Focus
TransducUon of GPR 68 adenovirus in HCN cells Coupling Hippocampal neurogenesis to brain PH through proneurogenic small. molecules that regulate proton sensing G protein-coupled receptors. ACS Chemical Neuroscience. ... Summary of two years’ training in UTSW. • Acquired exper@se in lab techniques: – Cell culture, RT-­?PCR, immunocytochemistry, protein blo^ng and perfusion xa@on of hearts, etc.
Post
ECMB-101: ANIMAL CELL TISSUE CULTURE UNIT 1: Introduction History, Biology of cell culture, Laboratory design and layout, equipments, aseptic condition, safety, bioethics and validation UNIT 2: Media Culture vessels, substrates, defined media supplements, serum free media, media preparation and sterilization UNIT 3: Various Cell Culture Primary culture, subculture and cell lines, cloning and selection, cell separation, characterization, differentiation, transformation and immortalization UNIT. 4: Techniques and Media Contamination, cryopreservation, quantification, cytotoxicity, special cell t...
Scientists propose benchmark for better replication of natural...
Though it has been known that culture techniques can affect what kind of cells pluripotent stem cells eventually become, no “gold standard” has yet been established to help scientists determine how the artificial environment can more closely replicate that found in a natural state (in vivo). “When you have culture conditions that more consistently match a non-artificial environment, you have the potential for a much better reflection of what is going on in actual human development,” said Kevin Huang, postdoctoral fellow in Fan’s lab and a lead author of the study.
4 November
Laboratory Science
Describe experience with the following techniques: iodinating proteins, cell cultures, RNA isolation and. measurements, protein assays, histology, fixing, embedding and sectioning tissue, samples for regular histology and immunocytochemistry. • ... What area of chemistry/public health/environmental issues are you most interested in? • What laboratory techniques are you familiar with? • This job requires a passion for “hands on” lab work. What makes you a good technician in the lab? •
Research in the Biological Sciences (RIBS)
· Learn some basic lab operations and techniques. · Learn how to design and carry out a research project. ... Can the worms still handle high salt? Using fluorescence reporters, students will monitor intracellular events that result from osmotic shock. · Cell Culture students will learn the basic steps in passaging and culturing cell lines. They will culture a mouse immune system B cell line and look at the effects of various drugs on the ability of the cells to proliferate.
record – LABS
LABS. Leonardo ABstracts Service. Menu.
31 August
Animal Cell Culture Lab.-Meiho University
::: Animal and human cell culture. laboratory. Persons in charge: Dr. Ray-Ling Huang. The term “cell culture” denotes the process by. which eukaryotic cells, especially from animals and. ... The. technique has been used in producing antiviral. vaccines, anticancer drugs and many other biotech. products. The lab has been put in service for. supporting the teaching and researching activities of. cell culture.
9 March
Why plant tissue culture?
Why plant tissue culture? There are a lot of different kinds of experiments we could do in a Plant Physiology lab, and we will do some traditional physiological experiments. Even before the course started, you probably imagined projects on photosynthesis, flowering or growth. ... Cleanliness, good sterile technique, and attention to keeping your airway clear is of paramount importance for success in any plant tissue culture! 4. MEDIA. Since plant tissue culture involves the in vitro culture of plants or cells in small containers, careful attention must be paid to the plants' nutritional needs.
Lab practical exam I
(2pts) 4. Demonstrate trypsinization technique.
Duan Lab | Regenerative Medicine | University of Nebraska...
The long-term goal of Dr. Duan’s lab (Biomaterials, Biofabrication and Biointerface Lab) is to create multi-scale complexity within engineered tissues to understand the fundamentals of cell-material interactions and mimic the native tissues for biomedical applications with a specific emphasis on tissue disease, tissue regeneration and stem cell bioengineering. ... Specifically, tissue engineering provides biologically inspired materials, culture techniques, and analytical tools that enable the recreation of humanized 3D diseased microenvironments in vitro.
11 February
New Page 2
1. The spread plate technique (see Figure below) - a mixture of cells or of a cell culture is spread out on the Agar surface of a Petri dish with the help of a flame-sterilized glass rod-made cell spreader (see Image below) - after incubation in an incubator over a defined time period - usually between 12 and 48 hours - the surface-dispersed cells. ... • The careful and scientific study of microorganism growth is an important part of microbiological lab routines; the growth of a population of microorganisms, e.g. a pure bacterial culture in growth medium, is usually studied by analyzing the growth curve of the microorganism under...
13 February
Phytoplankton Measuring and Culture Techniques
These signals are detected and analyzed. Advantages Can tell number, size, pigments of major divisions Can count picoplankton Some really expensive ones can sort out particular cells to culture or analyze! ... of ‘wild’ algae – many, many generations for further selection for traits that are beneficial in lab Many kinds of algae lose ‘wild’ traits in culture collections – e.g., growth pattern becomes unicellular instead of colonial Isolate your own algae Some algae are sensitive to collection techniques and do not do well if captured by net Some algae die quickly in a sample (even.
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Lab 2 : Cell culture lab | Faculty of Engineering and Natural...
Cell culture lab is aimed for the research of our academic staff, together with the MSc and PhD students. ... The lab is divided into animal cell culture lab and plant cell culture lab. These labs are very sterile rooms, equipped with UV lamps. Prev Next.
20 June
Cell Culture work
b) Split the cells 1. Start the biosafety cabinet and let it equilibrate for at least 3 min. Turn on UV light 2. Place the 1X trypsin into 37°C water bath for 3-5 min. 3. Label the new cell culture flasks with cell line type, passage number, date, and initials. ... Unless you do not have a need, in most cases the flask used in the split receives fresh media and is placed back in the incubator. Incubate flasks at 37°C. 16. Record split and passage number in your lab book. 17. If needed, change media in flask after 24 hours if dead cell debris is significant.
LAB EXERCISE: Microscopy and the Cell
EXERCISE 1. Basic Microscope Techniques. Materials clear ruler coverslips prepared slides: letter and crossed thread ... Materials microscope slides Amoeba culture. coverslips Paramecium culture. ... In this lab study, you will examine epithelial cells.
BIO Course Descriptions - Undergraduate Catalog 2011-2012...
This course covers theoretical and practical introduction to methods of animal cell culture necessary for research in biomedical or biotech labs and in the pharmaceutical industry. ... BIO 413 Elements of Immunology Laboratory (0-2-1). Prerequisite or Corequisite: BIO 412. This course covers immunochemical assays and cell culture techniques; preparation of single-cell suspensions and determination of cell viability; andidentification and quantification of antigen-antibody interactions.
18 May
Cell Culture - Toxicity Laboratory - Yasam Bilimleri...
DNA and RNA studies in cell culture. Apoptosis, necrosis studies. Fluorescent staining techniques and fluorescence microscopy studies. ... Cell culture - toxicity laboratory provides technical and scientific support for theses and projects. Available devices: Inverted Microscope.
18 July
Cell Adherence and Its Effect on Collagen
...are laboratory techniques widely used to detect small molecules and proteins within a cultured cell. ... Work in this project is dedicated to Linda M. Mercer, 1949—2010, Oxford Lab ... The nutrient rich culture showed rapid proliferation. Clusters were observed B)...
BIOL 201 - Cell & Molecular Biology
Cell Culture Intro to Biotech PCR. Lab Exercise 9 (due as specified by instructor). ... Assume lab exercises are due at the end of the lab period unless indicated differently in the syllabus or by your instructor during lab. B Introductory Labs: During this two-week period, you will meet your laboratory instructor, review some basic techniques and skills you should have learned in BIOL 111, and expand upon that initial foundation.
The College of St. Scholastica | Home Page
The College of St. Scholastica is an independent private college based in Duluth, Minnesota with campuses across the state. St. Scholastica offers undergraduate, graduate and certificate programs both online and on campus.
15 December
Characterization of Sterilization Techniques on a Microuidic...
Because cell cultures require a sterile environment, the device must be sterile as well. This study was designed to investigate the eects of common sterilization techniques on the diusive properties of the device. ... 4 H. L. T. Lee, P. Boccazzi, R. J. Ram, and A. J. Sinskey, Lab on a Chip 6, 1229 (2006). 5 C. B. Allen, B. K. Schneider, and C. W. White, American Journal of Physiology-Lung Cellular and Molecular Physi-ology 281, LA2 (2001). 6 T. M. Squires and S. R. Quake, Reviews of Modern Physics 77, 977 (2005).
DNA Extraction
DNA is extracted from human cells for a variety of reasons. With a pure sample of DNA you can test a newborn for a genetic disease, analyze forensic evidence, or study a gene involved in cancer. Try this virtual laboratory to perform a cheek swab and extract DNA from human cells.
20 September
Cell Biology (Biology 3403) Course Schedule
STUDENT LEARNING OUTCOMES: At the completion of this course a student will be able to: • Demonstrate a basic understanding of fundamental cell biology topics such as cell membranes and cell interactions, the flow of information in a cell, the flow of energy in a cell, and cell division • Discuss current scientific issues related to cell biology • Demonstrate basic laboratory techniques ... You should. read and study those sections that are covered during lecture in detail. Cell biology lab: The lab will comprise a substantial portion of your course grade. It is in your best interest to attend every lab.
Protocol for culturing of Osteosarcoma cell line: NCTC clone...
Ohara-Jasmin Lab Duc Tien May 2002. Protocol for culturing of Osteosarcoma cell ... F. Transfer contents to culture vessel and immediately add 10-15ml of warmed medium. ... B. Splitting adherent cells using cell scraper 1. Aspirate medium from the cell monolayer.
Cell culture lab
FOC?S LAB. Fabrication of Organoid and Complex ? (micro/nano)-scale biomimetic Surfaces Laboratory. Kidambi Lab.
25 April
SS12BMI - Laboratory Experiments
No white precipitate - gelatin hydrolysis +ve. 3.2- Culture Techniques for Anaerobic Bacteria. 3.2.1 Introduction Oxygen plays an important role in the metabolism of bacteria, as an electron acceptor. ... Measurement of cell mass In these techniques the actual numbers of cells are not counted, although estimates of the number can be made using a standard curve of cell mass vs cell number for that particular organism growing under similar conditions. Dry weight. The main limitation here is having enough cells to measure.
13 February
Coating plates & Primary Neuron Cell Cultures- sh-lab 9/2010
sh-lab 9/2010. Coating 24-well plates for Primary cell Cultures. 1) According to Ignatova, Kukekov i. Materials: 1. Polyornithin: Sigma cat# P3655 a. Stock: 10mg/ml in ddH20, keep frozen 100ul aliquots 2. Natural Mouse Laminin: Gibco/BRL cat#23017-015 a. Stock: 10-20ul aliquots frozen.
Publications | Denise Montell Lab | UC Santa Barbara
Myosin Type IV Myosins Neoplasm Invasiveness Neoplasm Metastasis Neoplasm Transplantation Neoplasms Neoplastic Cells, Circulating Neovascularization, Pathologic Nerve Tissue Proteins Neuregulins NIH 3T3 Cells Norethindrone Nuclear Proteins Nuclear Receptor Coactivator 3 Nucleic Acid Hybridization Oligonucleotide Array Sequence Analysis Oocytes Oogenesis Optical Imaging Optogenetics Organ Culture Techniques Organ Size Organ Specificity Organogenesis Osteoblasts ... alpha Transforming Growth Factors Tropomyosin Tumor Cells, Cultured Vascular Endothelial Growth Factor A Zygote.
9 June
Biotechnology (BIOT) < Johnson County Community College
Techniques include growth and maintenance of E. coli, gene cloning, DNA and protein electrophoresis protein purification and enzymatic and immunology assays. Lecture and laboratory exercises on the principles and practices of initiation, cultivation, maintenance, preservation of cell culture lines and applications will also be covered. 3 hrs lecture, 6 hrs. lab/wk.
18 April
The University of Jordan E-Learning Portal: Login to the site
Login here using your username and password (Cookies must be enabled in your browser).
9 April
Stem Cell Training Program | California State University...
397L Intro to Cell and Stem Cell Culture Lab or 477/L Cell and Tissue Culture and Lab (offered during program in summer and free). highly recommended 375 Current Issues in Regenerative Medicine (Upper Division GE). Minimum GPA of 3.0. ... Week-long intensive Stem Cell Techniques training course during internship (grant pays tuition). Research Presentations at UCLA and CSUN symposia, CIRM meetings, and international research conferences (grant travel funding pays). Community Outreach activities during internship.
15 February
Clinical Laboratory
LAB. CLS 523. Fundamentals of Analytical Techniques Laboratory. 3 Hours. Student laboratory with recitation addressing techniques and methodologies used in the clinical laboratory. Laboratory skills include laboratory math, quality control, pipetting, and instrumentation used in analysis of body fluids. ... A lecture course covering the theory underlying molecular techniques involving nucleic acids and mammalian cell culture.
Academic Courses
Credit: 4 (3 lecture, 3 lab) Theory and applications of cell culture techniques. Laboratory emphasis on the principles and practices of initiation, cultivation, maintenance, preservation of cell lines and applications. BITC 2441 Molecular Biology Techniques. Prerequisite: BITC 2411 or Department Approval. ... Credit: 3 (3 lecture, 0 lab) Study of the principles, techniques, and skills needed for college level scientific, technical, and business writing. FCEL 1302 Introduction to Fuel Cell Technology. Credit: 3 (2 lecture, 4 Lab) Types of fuel cells and other alternative energy fields.
Canary Center at Stanford Internship Projects | Canary Center...
Additionally, we are interested in understanding molecular mechanism that govern the self-renewal activity of adult stem cells and cancer stem cells. We use molecular biology techniques, cell culture based adult stem cell assay and in vivo tissue regeneration models of cancer. Faculty Mentor: Juergen K. Willmann, MD Internship Mentors: Ahmed El Kaffas, PhD, Alireza Akhbardeh. Internship Positions: 1 positions available, Undergraduates only Type of Laboratory Research: Dry lab.
4 December
Los Angeles Trade-Technical College | Laboratory quizzes
Microbial World & You (cont)/ Chemical Foundations of. 8/28 Life. Aseptic Techniques; Bacteria. 2 9/2 Labor Day (No Class). 9/4 Functional Anatomy of Prokaryotic & Eukaryotic Cells Negative Staining. ... 5 9/23 Exam 1, Microbial Metabolism (cont). Evaluate pure culture technique lab. Bacterial population count (enumeration; SPC)
Cell Biology (Bio 212): Syllabus and Schedule
Laboratories should not be missed. If you must miss a lab, it is your responsibility to contact your lab instructor, ideally ahead of time, to make arrangements to make up the work. Coming to class late is at best annoying and at worst highly disrespectful to your instructor and classmates. ... W 10/25 Primary literature reading: K+ channel Cell culture: basic techniques, Structure (Doyle, et al., Science, 1998) population curve F 10/27 Cell signaling: Receptors and Messengers (Ch.
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Aseptic transfer & pure culture techniques
Safety: · no eating or drinking in the lab! · Wash your hands with soap both ... TO AN AGAR PLATE CULTURE: see directions below on streak technique. ... STREAK PLATE You should see bacterial cells growing along streak lines and in isolated areas.
Safety concerns/lab techniques
Safety concerns/lab techniques. 1. A cold hot plate looks just like a hot hot plate. Be careful. ... Design an experiment to illustrate the shape and size of yeast cells. Materials: Yeast culture, pipet, microscope, microscope slide, coverslip, various vital stains. 4. Build models of organic molecules involved in Fermentation and Glycolysis. Submit the model and an accurate drawing to get credit.
Site Maintenance
Sorry for the inconvenience, but we’re performing some maintenance at the moment. We’ll be back online shortly! — The Team.
18 August
TTUHSC :: Cancer Center : Core Laboratories
This core laboratory serves as the Cell Culture and Xenograft Repository for the Childen's Oncology Group and also is the Texas Cancer Cell Repository (www.TXCCR.org). New cell lines and xenografts are initiated from both adults and children with cancer, expanded, characterized, and readied for distribution. The core can also provide cell lines that are obtained from other sources (if those lines can be subdistributed) to TTUHSC investigators.
29 April
Biology | 2016-17 Undergraduate Catalog
The laboratory work will include an examination of different types of cells, the cellular environment, cell culture, cellular bioenergetics and cell work, as well as an introduction to the instrumentation used to study cellular activities. ... The laboratory will provide experience in field and lab techniques used in the study of insects (3 hrs lecture — 3 hrs laboratory). Prerequisite: BIO 104 with a minimum grade of “C-” or better.
7 October
Isolation of pure cultures | B. Streak Plate Technique
Turbid solutions such as cell cultures interfere with light passage through the sample, so that less light hits the photocell than would if the cells were not there. Turbidimetric methods can be used as long as each individual cell blocks or intercepts light; as soon as the mass of cells becomes so large that some cells effectively shield other cells from the light, the measurement is no longer accurate. ... Procedure: (work in pairs). A. Spread Plate Technique. 1. Prepare serial dilutions of the broth culture as shown in the figure from a previous lab exercise (Isolation of Pure Cultures).
Inactive Content
Inactive Content. This page is in the process of being created or has temporarily been inactivated. If you have any questions please contact the following person...
2 February
BIO1094 – Cell & Tissue Culture – Sample Exam Questions
Question 1 (A) List three pieces of personal protective equipment in the cell culture laboratory. (B) Describe some precautions to avoid accidents in the cell culture laboratory. ... (C) Describe some obvious symptom of fungal contamination of cell culture. Question 3 (A) Describe a technique for determining cell viability after cryopreservation. (B) Describe how you would carry out serial passage of suspension cell culture.
Find 2017 Courses: Results
Subject Area. All Subject Areas ABORIG - Aboriginal Studies ACCTFIN - Accounting and Finance ACCTING - Accounting ACUCARE - Acute Care Medicine AGRIBUS - Agribusiness AGRIC - Agriculture & Natural Resource AGRONOMY - Agronomy ANAT SC - Anatomical...
16 November
The University of Melbourne, Australia - Australia's best...
Engagement at the University of Melbourne encompasses the mutually beneficial relationships the institution has with wider society. It connects our teaching, students and research work with communities – locally, nationally and globally – to help us achieve our academic aspirations and create economic, social and cultural value. This understanding of engagement is reflected in the University’s three engagement commitments
15 May
Students: Ricardo Gonzalez-Pinzon Lab
His project involved the recycling of polymers from the waste cell-phone plastics using solvent extraction techniques. Some of the skills that Sumant brings to our lab are: analytical intrument analysis (HPLC, UV spectroscopy, pyrolisis reactor, Anderson cascade impactor...) and lab techniques (cell culture, encapsulation, preparation of nano particles...). He is very interested in nano particles, their behavior and interfacial science.
1 November
BIO 495 (UT08) 3 - CELL CULTURE TECHNIQUES - Course...
You are here: Academics > Biology - BIOL > SPECIAL TOPICS > BIO 495 (UT08) 3 - CELL CULTURE TECHNIQUES > Course Information. ... Description. Requires permission of instructor. A lecture or laboratory course offered at the discretion of the Biology Department. Subject may focus on a current issue in biology, training in a specific research technique, or an area of biology that is of interest to a particular group of students.
10 April
Course Catalog | MOLECULAR BIOLOGY OF THE CELL
RECOMMENDED TEXT: Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, 6th Edition. R. Ian Freshney. Wiley-Blackwell, 2010. ISBN: 978-0-470-52812-9. This course focuses on both the fundamentals and practical aspects of mammalian cell culture and transgenic technologies. ... are responsible for learning new techniques, asking questions and working semi-independently by the end of each lab rotation.
Cell Culture Technique Tips
• Clean, fresh lab coats and glove should be worn at all times in the hood area, and removed as soon as the area is left. • UV light in hood should be on whenever hood is not in use. This means that all UV-sensitive materials (e.g., media) should be kept out of the hood, and shadowed from UV. ... stronger compounds (e.g. quaternary ammonium disinfectants) can be used for washing down surfaces • Doors to culture room should be kept closed at all times • Filters in pipettors should be changed regularly.
Lab-on-a-brane : a novel physiologically relevant planar
Essentially, their design enhances viability of cultured hepatocytes with functional bile canaliculi by controlling the cellular microenvironment at the micro-scale using lithography micro-patterning techniques. ... Lab-on-a-Chip Co-Culture Platforms Microfluidics organs-on-a-chip are typically single culture systems. For mimicking multi-organ or full-body-lab-on-chip, co-cultures are simulated by connecting single cell cultures in multi-well or multi-chamber configurations via fluid flow circuits16.
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